Alterations in oleanolic acid and sterol content in marigold (Calendula officinalis) hairy root cultures in response to stimulation by selected phytohormones

Abstract Hairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model analyzing effect various compounds on metabolism. In contrast to many other types in vitro cultures, hairy roots do not require external supply phytohormones medium. Consequently, growth regulators such as auxins cytokinins rarely used elicitors cultures; however, they can strongly influence defense responses. The aim this study was investigate two auxins: natural indole-3-acetic acid (IAA) synthetic 1-naphthaleneacetic (NAA), well cytokinins: kinetin 6-benzylaminopurine (BAP) at concentration 0.75 mg/L metabolism sterols triterpenoids Calendula officinalis roots. Auxins prevented accumulation triterpenoid saponins (oleanolic glycosides), while cytokinin BAP increased their by 17% release into culture medium factor 10. Other kinetins sterol levels 17%, level stigmasterol 15%, isofucosterol 7 times.